Types of Columns in HPLC
Types of Columns in HPLC
To classify liquid chromatography, there are several ways.However, also it can be adsorption chromatography, ion- exchange chromatography, If this bracket relies upon nature of the separation process and the stationary phase. In relation to the first type, two modes are defined relies on opposition to two phases, i.e. normal phase and reverse- phase chromatography, as mentioned types about 90 cover of all chromatography operations and for the separation of factors the column are used. Columns are generally made of SS, around 50 and 300 mm in length and within 2 and 5 mm, they're generally packed with a stationary phase of 3 to 10 µm size. Columns of the internal distance of lower than 2 mm are respectable regularly in the form of microbore HPLC columns.Types of packed gels
Silica gel
Silica gel is the most popular quilting material used. Silica gels are available in two types it's a globular and irregular shape, Spherically shaped gels are most generally used. There's a severance on the face of the silica gel used in liquid chromatography. By contains the pores, it gives a bigger face area than without holes. The most silica columns currently have globular types and small size patches have been developed. The 5μm is a most generally used size, but small size 1.5 to 3μm gel is also used in the HPLC column. Small gels are packed in small column casing and thus retention time is reduced.Polymer gel
Silica gels were always used in the before stage of HPLC development. But, the polymer- grounded column is getting well- liked. Polyethylene and polypropylene are generally known polymers. Like silica gel, polymer gel columns are available in veritably small patches.Other gel
In addition to silica and polymer gels, the gels used are natural substances like Cellulose, chitosan agroceae, and dextrin and pottery for illustration zirconia and hydroxyptite are used in liquid chromatography for the separation of analytes but is have veritably limited use.There are several types of columns used in the separation of factors with the help of the mobile phase, which is grounded on the separation mode used in liquid chromatography. Then some types of separation modes are mentioned.
Reversed- phase chromatography( RPC), Normal- phase chromatography( NPC), Hydrophilic commerce chromatography( HILIC), ion exchange chromatography( IEC), ligand exchange chromatography( LEC), ion rejection chromatography( IEC), Gel saturation chromatography( GPC), Gel Filtration Chromatography( GFC), Affinity chromatography, Chiral chromatography.
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