Types of Columns in HPLC
Types of Columns in HPLC To classify liquid chromatography, there are several ways.However, also it can be adsorption chromatography, ion- exchange chromatography, If this bracket relies upon nature of the separation process and the stationary phase. In relation to the first type, two modes are defined relies on opposition to two phases, i.e. normal phase and reverse- phase chromatography, as mentioned types about 90 cover of all chromatography operations and for the separation of factors the column are used. Columns are generally made of SS, around 50 and 300 mm in length and within 2 and 5 mm, they're generally packed with a stationary phase of 3 to 10 µm size. Columns of the internal distance of lower than 2 mm are respectable regularly in the form of microbore HPLC columns. Types of packed gels Silica gel Silica gel is the most popular quilting material used. Silica gels are available in two types it's a globular and irregular shape, Spherically shaped gels are most general...
